mouse anti vascular endothelial growth factor vegf  (Santa Cruz Biotechnology)

Journal: Regenerative Therapy

Article Title: Fabrication of Piezo1 protein encapsulated pressure-sensitive multifunctional hydrogel in modulating cellular response and wound healing in pressure ulcer conditions

doi: 10.1016/j.reth.2025.06.014

Figure Lengend Snippet: Determination of the angiogenic potential and gene expression of NHI 3T3 and HUVEC cell lines. (A–C) Denotes the number of nodes and segments of 3T3 cell lines and the representative images for tube formation assay. (D–F) Indicates the tube formation changes of HUVEC when treated with PP-MHs. (G & H) Represents the relative mRNA expressions of angiogenic genes (VEGF, FGE2, ADAM19, MM9) in NHI 3T3 and HUVEC cell lines. The data in each bar represents the mean ± SD of three independent samples. They were statistically significant, as indicated by the symbols ∗∗ for ( p < 0.01 ), and ∗∗∗ for ( p < 0.001 ).

Article Snippet: The membranes were incubated overnight at 4 °C following a blocking procedure with 5 % non-fat milk powder and subsequent treatment with rabbit anti-neutrophil elastase (29 kDa), rabbit anti-precursor transforming growth factor (TGF)-β 1/2/3 antibody, rabbit anti-matrix metalloproteinase (MMP)-12, goat anti-monocyte chemoattractant protein (MCP)-1 antibody, and mouse anti-vascular endothelial growth factor (VEGF) obtained from Santa Cruz Biotechnology.

Techniques: Gene Expression, Tube Formation Assay

Journal: Frontiers in Pharmacology

Article Title: Bletilla striata polysaccharide induces autophagy through PI3K/AKT signaling pathway to promote the survival of cross-boundary flap in rats

doi: 10.3389/fphar.2025.1544932

Figure Lengend Snippet: BSP upregulated the expression of VEGF and eNOS protein (A) Western blot was used to detect the expression of VEGF and eNOS protein. (B, C) Statistical chart of protein quantitative analysis. Compared with the control group, ***p < 0.001, ****p < 0.0001, compared with the BSP-M group, # p < 0.05, ### p < 0.001, #### p < 0.0001.

Article Snippet: Mouse anti-vascular endothelial growth factor (VEGF), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), PI3K, vascular endothelial cadherin (CDH5), rabbit anti- endothelial nitric oxide synthase (eNOS), AKT, vacuolar protein sorting 34 (VPS34), matrix metalloproteinase 9 (MMP9), microtubule-associated protein light chain 3 (LC3) antibody, HRP goat anti-mouse secondary antibody, HRP goat anti-rabbit secondary antibody (Proteintech Group, Inc.).

Techniques: Expressing, Western Blot, Control

Journal: Frontiers in Pharmacology

Article Title: Bletilla striata polysaccharide induces autophagy through PI3K/AKT signaling pathway to promote the survival of cross-boundary flap in rats

doi: 10.3389/fphar.2025.1544932

Figure Lengend Snippet: The inhibition of autophagy reverse the effect of BSP on blood vessels (A, B) Western blot was used to detecte the expression of VEGF,CDH5,MMP9 and eNOS protein. (C–F) Statistical chart of protein quantitative analysis. Compared with BSP-M group, **p < 0.01, ***p < 0.001, ****p < 0.0001, n = 6.

Article Snippet: Mouse anti-vascular endothelial growth factor (VEGF), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), PI3K, vascular endothelial cadherin (CDH5), rabbit anti- endothelial nitric oxide synthase (eNOS), AKT, vacuolar protein sorting 34 (VPS34), matrix metalloproteinase 9 (MMP9), microtubule-associated protein light chain 3 (LC3) antibody, HRP goat anti-mouse secondary antibody, HRP goat anti-rabbit secondary antibody (Proteintech Group, Inc.).

Techniques: Inhibition, Western Blot, Expressing